Zymopure plasmid miniprep kit. 2 mg of transfection grade plasmid DNA from E.
Zymopure plasmid miniprep kit. 8 and Abs260/230 ≥ 2.
Zymopure plasmid miniprep kit If they do, incubate at 37°C for 10-20 min and mix by inversion Aliquote solution to groups with solutions aliquoted to groups Page 7 of 7. com: Zymo Research D4209 ZymoPURE Plasmid Miniprep Kit, 50 Preps : Industrial & Scientific. coli alkaline lysis method with our The ZymoPURE™ Plasmid Miniprep Kit utilizes a spin column technique to isolate ultra-pure, endotoxin-free plasmid DNA, yielding up to 100 µg in less than 15 minutes. Part of the ZymoPURE™ Plasmid Kits collection, the ZymoPURE™ Plasmid Miniprep Kit features a spin column-based method for the purification of up to 100 µg of ultra-pure endotoxin-free plasmid DNA in less than 15 minutes. Contents Part of the ZymoPURE™ Plasmid Kits collection, the ZymoPURE™ Plasmid Miniprep Kit features a spin column-based method for the purification of up to 100 µg of ultra-pure endotoxin-free plasmid DNA in less than 15 minutes. Catalog No. coli using a traditional 3-buffer (P1, P2, P3) procedure that is simple, rapid, and reliable. Utilizing a modified alkaline lysis in conjunction with a patented binding system, the ZymoPURE II Maxiprep kit can process up to D4208T ZymoPURE Plasmid Miniprep Kit (10 Preps) Components: ZymoPURE P1 (Red) ZymoPURE P2 (Blue) ZymoPURE P3 (Yellow) ZymoPURE Binding Buffer ZymoPURE Wash 1 ZymoPURE Wash 2 with Ethanol Added ZymoPURE Elution Buffer. D4212 800 preps. The Zymoprep-96 is a simple and efficient yeast plasmid miniprep kit that is based on the classic E. Complete bacterial cell lysis, The ZymoPURE™ Plasmid Miniprep Kit features a spin column-based method for the purification of up to 100 g of ultra-pure endotoxin-free plasmid DNA in less than 15 minutes. El diseño único de columna de giro también proporciona retención de tampón cero y un bajo volumen de elución. 0 Recommended Sample Volume : ≤ 5. ZERO BIAS - scores, article reviews, protocol conditions and more ZymoPURE™ Plasmid Miniprep Kit Catalog Nos. The unique Part of the ZymoPURE™ Plasmid Kits collection, the ZymoPURE™ Plasmid Miniprep Kit features a spin column-based method for the purification of up to 100 µg of ultra-pure endotoxin-free plasmid DNA in less than 15 minutes. coli. Part of the ZymoPURE Plasmid Kits collection, the ZymoPURE II Plasmid Midiprep kit provides the fastest and simplest method available to efficiently isolate up to 400 µg of transfection grade plasmid DNA from E. 4C, D). 8 and Abs260/230 ≥ 2. As an added convenience, the ZymoPURE Plasmid Miniprep Kit contains colored buffers that permit error-free visualization and identification of complete bacterial cell lysis and neutralization. Part of the ZymoPURE™ Plasmid Kits collection, the ZymoPURE™ Plasmid Miniprep Kit features a spin column-based method for the purification of up to 100 μg of ultra-pure endotoxin-free plasmid DNA in less than 15 minutes. Fastest: Simple 20 minute Midipreps. Highest Yield: Purify up to 400 µg of plasmid DNA directly from a spin-column. • Innovative ZymoPURE™ technology enables elution of ultra-pure endotoxin-free plasmid DNA in as little as 25 µl. The unique spin-column design also provides zero Part of the ZymoPURE Plasmid Kits collection, the ZymoPURE Plasmid Miniprep Kit features a spin column-based method for the purification of up to 100 µg of ultra-pure endotoxin-free The ZymoPURE™ Plasmid Miniprep Kit features a spin column-based method for the purification of up to 100 µg of ultra-pure endotoxin-free plasmid DNA in less than 15 minutes. Highest Yield: Purify up to 100 μg of plasmid DNA in as little as 25 μl directly from a spin-column. The ZR Plasmid Miniprep-Classic is designed for efficient isolation of plasmid DNA from E. ZymoPURE™ Plasmid Miniprep Kit 10 Preps. us. ZymoPURE plasmid midiprep and ZymoPURE plasmid maxiprep kits from Zymo Research enable the isolation of up to 400 µg (midiprep) or 1. Bioz Stars score: 95/100, based on 1 PubMed citations. The following procedure should be performed at room temperature (15-30°C). • Purified plasmid DNA contains 50,000 times fewer endotoxins than industry leading minipreps. Z. 5-5 ml of bacterial culture in a clear 1. ZymoPURETM Plasmid Miniprep Kit from Zymo Research Centrifuge 0. Highlights • Purify up to 100 µg of plasmid DNA in as little as 25 µl directly from a spin-column. Select the department you want As an added convenience, the ZymoPURE Plasmid Miniprep Kit contains colored buffers that permit error-free visualization and identification of complete bacterial cell lysis and neutralization. RNA purification. 800 Preps. The ZymoPURE™ II Plasmid Midiprep Kit features a simple spin-column based method for the purification of up to 400 µg of transfection grade plasmid DNA in less than 20 minutes. 96-Well Non-Filtered Tips, LiHa Head The Zymoprep-96 Yeast Plasmid Miniprep kit provides all the necessary reagents for high-throughput plasmid isolation from S. pombe, and any fungi whose cell walls are susceptible to yeast lytic enzyme lysis. 20 Preps. coli using the fastest and easiest method on the market. DNA for transfection was purified by Zymopure Plasmid Miniprep Kit (Zymo), Zymopure Express Plasmid Midiprep Kit (Zymo), or Qiagen Plasmid Plus Midiprep Kit (Qiagen). ) Storage Temperature ZP Express™ Lysis Buffer1 210 ml Room Temp. Inquire ZymoPURE™ II Plasmid Maxiprep Kit 77001-468 10 preps Zymopure Plasmid Miniprep Kit, supplied by Zymo Research, used in various techniques. ) Storage Temperature ZymoPURE™ P11 (Red) 150 ml 2x 150 ml 4°C ZymoPURE™ P22 (Green) 150 ml 2x 150 ml Room Temp. Utilizing a modified alkaline lysis in conjunction with a patented binding system, the ZymoPURE II Midiprep kit can process up The ZymoPURE Plasmid Miniprep Kit features a spin column-based method for the purification of up to 100 µg of ultra-pure endotoxin-free plasmid DNA in less than 15 minutes. coli alkaline lysis method but using Zymolyase as the first solution. 3). Highest Yield: Purify up to 100 μg of 05/28/2021 Kit Components Product code Description D4209,D4210,D4211,D4212 ZymoPURE Plasmid Miniprep Kit Components: D4200-1-3, D4200-1-13 ZymoPURE P1 (Red) D4200-2-3, D4200-2-13 ZymoPURE P2 (Green) D4200-3-3, D4200-3-13 ZymoPURE P3 (Yellow) D4200-4-3, D4200-4-14 ZymoPURE Binding Buffer Part of the ZymoPURE™ Plasmid Kits collection, the ZymoPURE™ Plasmid Miniprep Kit features a spin column-based method for the purification of up to 100 µg of ultra-pure endotoxin-free plasmid DNA in less than 15 minutes. The unique ZymoPURE™ methodology removes the need for slow This method is spin column-based and purifies up to 100 \(\mu g\) of ultra-pure endotoxin-free plasmid DNA in less than 15 minutes. ) D4203 (20 preps. Utilizing a modified alkaline lysis in conjunction with a patented binding system, the ZymoPURE II Midiprep kit can process up to 50 ml of culture in less than 18 minutes. pombe and any fungi whose cell walls are susceptible to yeast lytic enzyme lysis. D4209 D4210 D4211 D4212 D4208T. The ZymoPURE Miniprep kit utilizes a modified alkaline lysis system in conjunction with a novel binding system. The ZymoPURE™ Plasmid Miniprep Kit features a spin column-based method for the purification of up to 100 µg of ultra-pure endotoxin-free plasmid DNA in less than 15 minutes. ZP Express™ Neutralization Buffer2 260 ml 4 – 8 °C ZP Express™ Binding Buffer 260 ml Room Temp. Todos los demás reactivos se almacenarán a temperatura I am using the Zymopure plasmid miniprep kit and I have repeated my extractions at least 6-7 times now. Highest Yield: Purify up to 100 µg of plasmid DNA in as little as 25 µl directly from a spin-column. The The ZymoPURE™ Plasmid Miniprep Kit features a spin column-based method for the purification of up to 100 µg of ultra-pure endotoxin-free plasmid DNA in less than 15 minutes. 76. 5 ml tube at full speed for 15- The ZymoPURE™ Plasmid Miniprep Kit features a spin column-based method for the purification of up to 100 µg of ultra-pure transfection-grade plasmid DNA in less than 15 minutes. albicans and S. Put P3 on ice 3. Description Description. Supplier: Zymo Research. Part of the ZymoPURE Plasmid Kits collection, the ZymoPURE Plasmid Miniprep Kit features a spin column-based method for the purification of up to 100 µg of ultra-pure endotoxin-free plasmid DNA in less than 15 minutes. A. If you are unsure about your plasmid DNA needs, then the ZymoPURE Miniprep Kit is a good starting point because of its versatility. 5-2. 13 USD. The result is plasmid DNA suitable for transfection, restriction endonuclease digestion, bacterial transformation, PCR amplification, and DNA sequencing. ZymoPURE 96 Plasmid Miniprep Kit Rapidly perform 96 Transfection-Grade Plasmid Minipreps Simultaneously. 5 mL tube at full speed for 15 – 20 seconds in a microcentrifuge. D4211 . The provided wash plate also significantly reduces plasmid DNA Part of the ZymoPURE Plasmid Kits collection, the ZymoPURE Plasmid Miniprep Kit features a spin column-based method for the purification of up to 100 µg of ultra-pure endotoxin-free plasmid DNA in less than 15 minutes. The ZymoPURE Miniprep kit As an added convenience, the ZymoPURE™ 96 plasmid miniprep kit contains colored buffers that permit error-free visualization and identification of complete bacterial cell lysis and neutralization. 2 mg of transfection -grade plasmid DNA directly from E. D4208, D4209, D4210, D4211 & D4212 Centrifugation Protocol Before Starting: Incubate ZymoPURE™ P3 on ice for 30 minutes before use. Transfection-Grade: 50,000 times fewer endotoxins ZymoPURE plasmid midiprep and ZymoPURE plasmid maxiprep kits from Zymo Research enable the isolation of up to 400 µg (midiprep) or 1. 04 EU/µg of plasmid DNA meets the FDA limit for vaccines Product VWR Part Number Size ZymoPURE™ Plasmid Miniprep Kit 77001-478 50 preps. coli culture. N. Great prices & free shipping on orders over $50 when you sign in or sign up for an account. 1. (ZymoPURE Plasmid Miniprep Kit) Applicable For: Transfection, transformation, sequencing, restriction endonuclease digestion, in vitro transcription, in vivo studies, genome editing, and other sensitive applications. Increasing the amount of culture processed leads to higher total yield of plasmid DNA if kit parameters are adjusted accordingly to accommodate higher ZymoPURE™ Plasmid Miniprep Kit 10 Preps. Request a free sample: h The ZymoPURE™ II Plasmid Midiprep Kit features a simple spin-column based method for the purification of up to 400 µg of transfection grade plasmid DNA in less than 20 minutes. Discard supernatant. 2 mg of transfection grade plasmid DNA in less than 18 minutes. ZymoPURE™ Wash 1 3 x 55 ml Room Temp. The recovered plasmid DNA is highly concentrated (up to 6 µg/µl), endotoxin-free and transfection-ready. Finney states that the components are well-designed, though one piece is too large to fit in standard-size conical tube The ZymoPURE 96 Plasmid Miniprep Kit features a high-throughput method for the purification of up to 100 µg of ultra-pure transfection-grade plasmid DNA using a vacuum manifold or centrifuge. Request A Quote. Format : 96-Well Plate Processing Time : ≤ 60 min Purity : Typical Abs260/280 ≥ 1. pombe, and other fungi species sensitive to yeast lytic enzymatic digestion (Zymolyase™). The Zyppy-96 Plasmid MagBead Miniprep is the fastest high-throughput (96-well), Pellet-Free plasmid DNA kit available for efficient isolation of plasmid DNA from E. 2. ZymoPURE™ Plasmid Miniprep Kit Rapid purification of transfection-grade plasmid DNA from up to 5 ml of overnight E. ZymoPURE 96 Plasmid Miniprep Kit High-throughput purification of up to 100 µg of transfection-grade plasmid DNA. D4209, D4210, D4211 & D4212 (Patent Pending) Highlights Fast and reliable purification of up to 100 µg of transfection-grade plasmid DNA using a spin-column. The unique ZymoPURE methodology removes ZymoPURETM Plasmid Miniprep Kit D4210 100 preps ≤ 15 min 5 ml ≥ 25 µl 100 µg ≤ 1. Highlights • Perform 96 plasmid minipreps The ZymoPURE™ Miniprep Kit from Zymo Research enables the isolation of up to 100 µg of endotoxin, salt, protein and RNA-free transfection quality plasmid DNA from E. ZymoPURE™ Plasmid Miniprep D4210 Kit Size Temperature D4209 50 preps. • Plasmid Purity: Plasmid is recovered in water or TE. Highlights. view : ZymoPURE Plasmid MiniPrep Kit D4210 · Zymo Research popular product: D4210. 77001-478KT 104. D4200 D4201 ZymoPURE™ II Plasmid Maxiprep Kit 10 Preps. This plasmid DNA kit features a modified alkaline lysis system The recovered plasmid DNA is highly concentrated (up to 3 µg/µl), EndoZero, vaccine-grade, and transfection-ready. We further revealed that the use of DM mixing options has a great advantage for Part of the ZymoPURE Plasmid Kits collection, the ZymoPURE Plasmid Miniprep Kit features a spin column-based method for the purification of up to 100 µg of ultra-pure endotoxin-free plasmid DNA in less than 15 minutes. Optimized miniprep protocol for ZymoPURE Plasmid Miniprep Kit miniprep. ZymoPUR The ZymoPURE™ Plasmid Miniprep Kit features a spin column-based method for the purification of up to 100 g of ultra-pure endotoxin-free plasmid DNA in less than 15 minutes. coli cell lysates using a procedure that is simple, rapid, user-friendly, and reliable compared to the products offered by the competition. Nota. The unique spin column facilitates Zymoprep™ II Kit 1 GENERAL INFORMATION Specificity: S. Make sure that the P2 and Binding buffer have no precipitates. Innovative ZymoPURE™ binding technology enables elution of the highest concentration of plasmid DNA Strep-Spin Protein Miniprep Kit™ The easiest way to purify Strep-tagged proteins The Strep-Spin Protein Miniprep Kit™ provides fast purification technology for Strep-tagged proteins. ZymoPURE™ technology uses a modified alkaline lysis This method is spin column-based and purifies up to 100 \(\mu g\) of ultra-pure endotoxin-free plasmid DNA in less than 15 minutes. Plasmid DNA The ZymoPURE Plasmid Miniprep Kit features a spin column-based method for the purification of up to 100 µg of ultra-pure endotoxin-free plasmid DNA in less than 15 minutes. High-throughput purification of transfection-grade plasmid DNA from up to 5 ml of overnight E. ZymoPURE™ Binding Buffer 150 ml 2x 150 ml Room Temp. Page 1/10 Safety ZymoPURE™ Plasmid Midiprep Kit Catalog Nos. The unique spin-column design also provides zero The ZymoPURE ™ Plasmid Midiprep kit features a spin column-based method for the purification of up to 300 µg of high-quality plasmid DNA in less than 18 minutes. The ZP Express™ Lysis Buffer is simply added directly to the culture and The ZymoPURE™ II Plasmid Maxiprep Kit features a simple spin-column based method for the purification of up to 3 mg of transfection grade plasmid DNA in less than 18 minutes. cerevisiae, C. It features a modified alkaline lysis protocol together with Zymo-Spin technology to yield high quality plasmid DNA in minutes. ZymoPURE™ technology uses a modified alkaline lysis method and features ZymoPURE-Express Plasmid Midiprep Kitは、集菌・再懸濁操作の必要がありません。 また、ZymoPURE Plasmid Miniprep Kitは96ウェルプレートフォーマットの製品もあります。 ※ 本製品は研究用です。研究用以外には使用できません。 The ZR Plasmid Miniprep™-Classic kit is designed for efficient isolation of plasmid DNA from E. albicans, S. * Endotoxins <0. coli culture using a vacuum manifold or centrifuge. Binding Capacity: 100 µg Culture Input: ≤ 5 mL Elution Volume: 25 µl Endotoxin Levels: ≤ 1 EU/µg /> Equipment: Microcentrifuge and/or vacuum manifold<br /> Format: Spin Column <br /> The ZymoPURE Plasmid Miniprep Kit features a spin column-based method for the purification of up to 100 µg of ultra-pure endotoxin-free plasmid DNA in less than 15 minutes. page 4 - 5 ZymoPURE™ Plasmid Miniprep Kit & ZymoPURE™ II Plasmid Midi, Maxi, & Gigaprep Kits Quickly isolate large constructs (~200 kb) using a spin-column. indd ZymoPURE™ Plasmid Miniprep D4210 Kit Size Temperature D4209 50 preps. Transfection-grade plasmid DNA from a miniprep ZymoPURE™ SizePlasmid Miniprep . com/pages/zymopure?utm_source %PDF-1. Inquire ZymoPURE™ II Plasmid Midiprep Kit 77001-396 25 preps. Select the department you ZymoPURE II Plasmid Isolation kits provide the fastest and simplest method available to efficiently isolate transfection quality plasmid DNA from E. As an added convenience, the ZymoPURE II Plasmid Midiprep kit contains colored buffers that permit error-free visualization and identification of complete bacterial cell lysis and neutralization. Utilizing a modified alkaline lysis in conjunction with a patented binding system, the ZymoPURE II Gigaprep kit can process up to The ZR Plasmid Miniprep-Classic is designed for efficient isolation of plasmid DNA from E. 00. 100 preps. D4208T D4309 D4210 D4211 D4212 20 Minute Endotoxin-Free Midi & Maxipreps ZymoPURE™ II Plasmid Prep Kits Size Catalog No. Binding Capacity: 100 µg Culture Input: ≤ 5 mL Elution Volume: 25 µl Endotoxin Levels: ≤ 1 EU/µg /> Equipment: Microcentrifuge and/or vacuum manifold<br /> Format: Spin Column <br /> Order Zymo Research ZymoPURE Plasmid Miniprep Kit, 400 Preps, ZD4211 at Zoro. com: Zymo Research D4209 ZymoPURE Plasmid Miniprep Kit, 50 Preps : Industrial & Scientific Skip to main content. Categories: Brands, Plasmid, Zymo Research. ZymoPURE™ Wash 2 (Concentrate) 3 x 23 ml Room Temp. Part of the ZymoPURE™ Plasmid Kits collection, the ZymoPURE™ Plasmid Miniprep Kit features a spin column-based method for the purification of up to ZymoPURE™ Plasmid Miniprep Kit Rapid purification of transfection-grade plasmid DNA from up to 5 ml of overnight E. Zymo Research: 100 preparations: £239. The unique ZymoPURE methodology removes (ZymoPure Plasmid Miniprep Kit) Nota. coli in less than 20 minutes. Sign Up & Save Take 10% off your next order when Amazon. 96-Well Non-Filtered Tips, LiHa Head ZymoPURE™ Plasmid Miniprep Kits, Zymo Research Supplier: Zymo Research. to OSHA HCS Printing date 07/18/2024 Reviewed on 03/15/2021 The ZymoPURE™ Plasmid Miniprep Kit features a spin column-based method for the purification of up to 100 µg of ultra- pure transfection-grade plasmid DNA in less than 15 minutes. ZymoPURE™ Plasmid Miniprep Kit : 10 Preps. Simply add the uniquely formulated 7X Lysis Buffer directly to your bacterial culture, neutralize, and then purify with 12/14/2021 Kit Components Product code Description D4213 ZymoPURE - Express Plasmid Midiprep Kit (25 preps) Components: D4213-1-210 ZP Express Lysis Buffer D4213-3-260 ZP Express Binding Buffer D4200-5-20, D4200-5-55 ZymoPURE Wash 1 D4200-6-10 ZymoPURE Wash 2 (Concentrate) D4200-7-1, D4200-7-6 ZymoPURE Elution Buffer. Nucleic Acid Reagents Nucleic Acid Purification Kits and Reagents. Compatible on a microcentrifuge or vacuum manifold for the purification of plasmid DNA. 2 mg of transfection grade plasmid DNA from E. ZymoPURE™ P3 (Yellow) 13 ml 2x 13 ml 100 ml 210 ml Room Temp. The vacuum pump should be a single or double-staged unit capable of producing up to 300 mm Hg at the vacuum manifold. The Zymoprep Yeast Plasmid Miniprep I is a simple and efficient yeast plasmid miniprep that is based on the E. com: Zymo Research D4208T ZymoPURE Plasmid Miniprep Kit, 10 Preps : Industrial & Scientific. Compare this item Store at -80°C ZymoPURE plasmid miniprep kit, D4211 (400 preps) 1. ZymoPURE™ 96 Plasmid Miniprep Kit The ZymoPURE™ 96 Plasmid Miniprep Kit features a high-throughput method for the purification of up to 100 µg of ultra-pure transfection-grade plasmid DNA using a vacuum manifold or centrifuge. Protocol: SDS (MSDS): ZymoPURE Plasmid Miniprep Kit quantity. ZymoPURE™ Plasmid Miniprep Kit Catalog Nos. Featuring ZymoPURE II technology along with a modified alkaline lysis system and novel binding chemistry, these kits can isolate up to 400 µg of vaccine/transfection grade, salt, Part of the ZymoPURE™ Plasmid Kits collection, the ZymoPURE™ Plasmid Miniprep Kit features a spin column-based method for the purification of up to 100 µg of ultra-pure endotoxin-free plasmid DNA in less than 15 minutes. ZymoPURE Plasmid Miniprep Kit: 50 Preps: Documents. Highlights • Purify up to 100 µg of plasmid DNA in as little Perform plasmid midipreps in only 16 minutes using a simple spin-column protocol. Innovative ZymoPURE™ binding technology enables elution of the highest concentration of plasmid DNA 12/08/2022 Kit Components Product code Description D4209,D4210,D4211,D4212 ZymoPURE Plasmid Miniprep Kit Components: D4200-1-3, D4200-1-13 ZymoPURE P1 (Red) D4200-2-3, D4200-2-13 ZymoPURE P2 (Blue) D4200-3-3, D4200-3-13 ZymoPURE P3 (Yellow) D4200-4-3, D4200-4-14 ZymoPURE Binding Buffer The ZymoPURE™ Plasmid Miniprep Kit features a spin column-based method for the purification of up to 100 µg of ultra-pure endotoxin-free plasmid DNA in less than 15 minutes. D4209, D4210, D4211 & D4212 (Patent Pending) Highlights • Fast and reliable purification of up to 100 µg of transfection-grade plasmid DNA using a spin-column. Dvec00 Zymopure Plasmid Miniprep Kit Zymo Research, supplied by Zymo Research, used in various techniques. 100 preparations. D4211 400 preps. Learn More According to densitometry analysis, the chromosomal fraction found in the plasmids was 60 ± 5 pg for the QIAGEN Plasmid Midi Kit, 120 ± 10 pg for the ZymoPURE™ II Plasmid Midiprep Kit, 105 ± 10 for the DM miniprep and 130 ± 10 for the SM miniprep (Fig. The ZymoPURE™ II Plasmid Midiprep Kit features a simple spincolumn based - method for the purification of up to 1. Contents Miniprep Kits New England Biolabs. Moreover, the isolated plasmid DNA is ultra-pure Applicable For: Transfection, transformation, sequencing, restriction endonuclease digestion, in vitro transcription, in vivo studies, genome editing, and other sensitive applications. 5 – 5 mL of bacterial culture in a clear 1. Go Back Tecan Genesis Freedom, EVO and Miniprep Tips. coli using Zymo-Spin column purification technology. The ZymoPURE – Express™ Plasmid Midiprep kit utilizes a patented alkaline lysis method for purifying up to 1. The eluted plasmid DNA is EndoZero and ready for immediate use in the most sensitive applications. D4208T D4209 : D4210 . Figure 1. coli culture without conventional cell-pelleting and resuspension steps. us Delivering to Lebanon 66952 Update location Industrial & Scientific Select the department you want to search in Search Amazon PDF-1. As an added convenience, the ZymoPURE II Plasmid Maxiprep kit contains colored buffers that permit error Part of the ZymoPURE Plasmid Kits collection, the ZymoPURE Plasmid Miniprep Kit features a spin column-based method for the purification of up to 100 µg of uLtra-pure endotoxin-free plasmid DNA in less than 15 minutes. Innovative ZymoPURE™ technology enables elution of ultra-pure endotoxin-free plasmid DNA in as little as 25 µl. 100 Preps. Purify up to 1. Delivering to Lebanon 66952 Update location Industrial & Scientific. The kit provides all the necessary reagents for plasmid isolation from S. Page 1/7 Safety Data Sheet acc. ZymoPURE™ Plasmid Miniprep Kits, Zymo Research. The recovered plasmid DNA is highly concentrated (up to 3 µg/µl), EndoZero, vaccine-grade, and transfection-ready. 77001-478 77001-480 77001-482 77001-484 76211-514. ZymoPURE - Express™ Plasmid Midiprep (Kit Size) D4213 (25 preps. Skip to main content. 05/28/2021 Kit Components Product code Description D4209,D4210,D4211,D4212 ZymoPURE Plasmid Miniprep Kit Components: D4200-1-3, D4200-1-13 ZymoPURE P1 (Red) D4200-2-3, D4200-2-13 ZymoPURE P2 (Green) D4200-3-3, D4200-3-13 ZymoPURE P3 (Yellow) D4200-4-3, D4200-4-14 ZymoPURE Binding Buffer The ZymoPURE™ II Plasmid Gigaprep Kit features a simple spincolumn based - method for the purification of up to 25 mg of transfection grade plasmid DNA in less than 45 minutes. ZymoPURE™ technology uses a modified alkaline lysis Part of the ZymoPURE™ Plasmid Kits collection, the ZymoPURE™ Plasmid Miniprep Kit features a spin column-based method for the purification of up to 100 µg of ultra-pure endotoxin-free plasmid DNA in less than 15 minutes. 0 mL Size Range : Up to ~200 kb Yield : Up to 100 µg per preparation, depending on Zyppy Plasmid Miniprep Kit, 800 Preps $ 902. Part of the ZymoPURE™ Plasmid Kits collection, the ZymoPURE™ Plasmid Miniprep Kit features a spin column-based method for the purification of up to 100 µg of ultra-pure endotoxin-free plasmid DNA Part of the D42 ZymoPURE™ Plasmid Kits collection, the D42 ZymoPURE™ Plasmid Miniprep Kit features a spin column-based method for the purification of up to 100 µg of ultra-pure endotoxin-free plasmid DNA in less than 15 minutes. El reactivo P1 es sensible a la temperatura debido a que la RNasa está presente, y debe mantenerse en la nevera o en hielo en todo momento. ZymoPURE™ filter plates are also included for rapid clearing of the lysate and the unique Zymo-Spin P-96 plate design allows the binding step to The ZymoPURE™ II Plasmid Midiprep Kit features a simple spin-column based method for the purification of up to 400 µg of transfection grade plasmid DNA in less than 20 minutes. El kit ZymoPURE Plasmid Miniprep cuenta con un método basado en columnas de giro para la purificación de hasta 100 µg de ADN plasmídico libre de endotoxinas ultra puro en menos de 15 minutos. Add 250 µL of ZymoPURETM P1 (Red) to the bacterial cell pellet and resuspend completely by vortexing or pipetting. view : ZymoPURE Plasmid MiniPrep Kit D4211 · Zymo Research: D4211. Pick 10 colonies and prepare 2 mL overnight cultures with corresponding antibiotic. Delivering to Nashville 37217 Update location Industrial & Scientific. coli in less than 15 minutes. Joel Finney from Duke University reviewed Zymo Research's ZymoPURE™ Plasmid Midiprep Kit and found that it has fewer steps and takes less time than other midikits they have used. Amazon. Certificates. The unique ZymoPURE methodology removes the need for slow 04/04/2023 Kit Components Product code Description D4214, D4215 ZymoPURE 96 Plasmid Miniprep Kit Components: D4200-1-3, D4200-1-13 ZymoPURE P1 (Red) D4200-2-3, D4200-2-13 ZymoPURE P2 (Blue) D4200-3-3, D4200-3-13 ZymoPURE P3 (Yellow) D4200-4-3, D4200-4-14 ZymoPURE Binding Buffer The ZymoPURE Plasmid Miniprep Kit features a spin column-based method for the purification of up to 100 µg of ultra-pure endotoxin-free plasmid DNA in less than 15 minutes. #D4209). ZymoPURE™ P3 (Yellow) 150 ml 2x 150 ml Room Temp. 2 mg of highly concentrated plasmid DNA directly ZymoPURE™ 96 Plasmid Miniprep Kit . 0 and the 260/230 The Zyppy Plasmid Miniprep Kit is a plasmid DNA kit that features a pellet-free modified alkaline lysis system that bypasses the normal cell-pelleting and resuspension steps common to classical plasmid preparation procedures. About this item. ZymoPURE Plasmid Miniprep; ZymoPURE II Plasmid Midiprep and Maxiprep; Zymoclean Gel DNA Recovery Kit; Zyppy Plasmid Miniprep; Invitrogen PureLink Plasmid Prep Kit; Macherey-Nagel: NucleoBond Xtra Midi EF, NucleoSpin; NEB Monarch; Takara Bio: NucleoSpin Plasmid; GoTaq Green 2x Master Mix; RedTaq polymerase Kit; Omega Bio-tek E. As an added convenience, the ZymoPURE – Express Plasmid Midiprep kit contains colored buffers that permit error-free visualization and identification of complete bacterial Watch this video of how to easily purify up to 100 µg of plasmid DNA in less than 20 minutes with the ZymoPURE Plasmid Miniprep Kit. ZymoPURE™ Plasmid Miniprep Kits, Zymo Research . Transfection-Grade: 50,000 times fewer endotoxins Buffer P1 (Red) is designed to be used with our ZR Plasmid Miniprep - Classic kit, which is used to efficiently isolate ultra-pure plasmid DNA from E. 12/08/2022 Kit Components Product code Description D4200 & D4201 ZymoPURE II Plasmid Midiprep Kit, 25 preps & 50 preps Components: D4200-1-3, D4200-1-13 ZymoPURE P1 (Red) The ZymoPURE – Express Plasmid Midiprep kit’s fast and simple protocol enables the recovery of highly concentrated transfection-grade plasmid DNA in just 15 minutes. Part of the ZymoPURE Plasmid Kits collection, the ZymoPURE II Plasmid Midiprep kit provides the fastest and simplest method available to efficiently isolate up to 1. The Plasmids were constructed using NEBuilder HiFi DNA Assembly or USER cloning (New England Biolabs) and purified with the Zyppy Plasmid Miniprep Kit (Zymo). The unique ZymoPURE methodology removes If you want to scale up your plasmid purification, try using a kit designed for high inputs of culture, such the ZymoPURE II Midipreps and Maxipreps, which can process more culture. Tel: +44 (0)1223 316 855; register; The ZymoPURE II Plasmid Midiprep kit provides the fastest and simplest method available to efficiently isolate up to 400 µg of transfection grade plasmid DNA from E. The procedure is based on a novel Strep-Tactin® XT Superflow ® resin which binds efficiently to Twin-Strep-tag, as well as single Strep-tag® Part of the ZymoPURE Plasmid Kits collection, the ZymoPURE II Plasmid Gigaprep kit provides the fastest and simplest method available to efficiently isolate up to 25 mg of transfection grade plasmid DNA from E. Centrifuge 0. ZymoPURE™ II Plasmid Midiprep Kit 25 Preps. 50 preparations. D4200 & D4201 (Patent Pending) Highlights The fastest spin-column based procedure for purifying up to 300 µg of ultra-pure endotoxin-free transfection-grade plasmid DNA. Utilizing a modified alkaline lysis in conjunction with a novel binding ZymoPURE™ II Plasmid Maxiprep Kit (Kit Size) D4202 (10 preps. Fast and reliable purification of up to 100 µg of transfection-grade plasmid DNA using a spin-column; Part of the ZymoPURE Plasmid Kits collection, the ZymoPURE Plasmid Miniprep Kit features a spin column-based method for the purification of up to 100 µg of ultra-pure endotoxin-free plasmid DNA in less than 15 minutes. The ZymoPURE Plasmid Miniprep Kit features a spin column-based method for the purification of up to 100 ug of ultra-pure endotoxin-free plasmid DNA in less than 15 minutes. This results in higher yields, higher Explore ZymoPURE™ plasmid purification and simplify the preparation of transfection-grade plasmid DNA: https://www. Conclusions By using a modified protocol, twice as much low-copy number plasmid DNA can be purified in approximately 25 minutes with the ZymoPURE™ II Plasmid Maxiprep Kit. 50 Preps. ZymoPure Plasmid Miniprep video. (ZymoPURE Plasmid Miniprep Kit) Part of the ZymoPURE™ Plasmid Kits collection, the ZymoPURE™ Plasmid Miniprep Kit features a spin column-based method for the purification of up to 100 μg of ultra-pure endotoxin-free plasmid DNA in less than 15 minutes. page 2 - 4 ZymoPURE II Plasmid Prep Kits Endotoxin-free plasmid Go Back Tecan Genesis Freedom, EVO and Miniprep Tips. The unique spin-column design also provides zero buffer retention and a low elution volume. Vea el video de Youtube para preguntas sobre el protocolo Miniprep. The unique spin-column design also provides zero The ZymoPURE™ Plasmid Miniprep Kit features a spin column-based method for the purification of up to 100 µg of ultra- pure transfection-grade plasmid DNA in less than 15 minutes. Features polypropylene construction and contains a unique silica-based matrix that allows purification of up to 100 µg of plasmid DNA in ≥ 25 µl eluate when used in combi Amazon. Bioz Stars score: 86/100, based on 1 PubMed citations. D4208T D4309 D4210 D4211 D4212 16 Minute Endotoxin-Free Midi & Maxipreps ZymoPURE™ II Plasmid Prep Kits Size Catalog No. Make back up cultures by decanting the tubes and refill with LB and corresponding ZymoPURE Plasmid MiniPrep Kit D4209 · Zymo Research: D4209. 96-Well Non-Filtered Tips, LiHa Head Part of the ZymoPURE™ Plasmid Kits collection, the ZymoPURE™ Plasmid Miniprep Kit features a spin column-based method for the purification of up to 100 µg of ultra-pure endotoxin-free plasmid DNA in less than 15 minutes. 2 mg (maxiprep) of salt, protein and RNA-free vaccine/transfection quality plasmid DNA from E. It features a modified alkaline lysis protocol together with a unique Fast Spin Part of the ZymoPURE Plasmid Kits collection, the ZymoPURE Plasmid Miniprep Kit features a spin column-based method for the purification of up to 100 µg of ultra-pure endotoxin-free plasmid DNA in less than 15 minutes. RNA Clean & Concentrator-5 (5 preps) – R1015S The RCC is for rapid clean-up of RNA from any enzymatic reaction. 0 EU/µg D4211 400 preps D4212 800 preps ZymoPURE™ 96 Plasmid Miniprep Kit D4214 2 x 96 preps ≤ 60 min 5 ml ≥ 125 µl 100 μg ≤ 1. The unique vacuum plate design provides zero buffer retention and low elution volumes. The eluted plasmid DNA is ready for immediate use, avoiding the need for subsequent precipitation steps. Life Sciences. Fastest and simplest method to isolate endotoxin-free plasmid DNA. 5 %µµµµ 1 0 obj >>> endobj 2 0 obj > endobj 3 0 obj >/XObject >/ProcSet[/PDF/Text/ImageB/ImageC/ImageI] >>/MediaBox[ 0 0 612 792] /Contents 4 0 R/Group Component in the ZymoPURE Plasmid Miniprep kit (Cat. zymoresearch. While the yield is okay (around 350ng), the 260/280 ratio is around 1. La tecnología ZymoPURE utiliza un ZymoPURE™ Plasmid Miniprep Kit Catalog Nos. Storage ZymoPURE™ P11 (Red) 13 ml 2x 13 ml 100 ml 210 ml 4°C ZymoPURE™ P22,3 (Green) 13 ml 2x 13 ml 100 ml 210 ml Room Temp. Maxiprep kit compared to two separate kits from Supplier Q. 4 %âãÏÓ 434 0 obj ZymoPURE Plasmid Miniprep ZymoPURE II Plasmid Midiprep and Maxiprep Zymoclean Gel DNA Recovery Kit Zyppy Plasmid Miniprep Invitrogen PureLink Plasmid Prep Kit Macherey-Nagel: NucleoBond Xtra Midi EF, NucleoSpin NEB Monarch Takara Bio. The unique Shop Zymo Research Corporation ZymoPURE Plasmid Miniprep Kits at Thomas Scientific, your trusted partner in Science. 400 Preps. Zymo Research: 50 preparations: £130. 0 EU/µg D4215 4 x 96 preps . The eluted plasmid DNA is endotoxin- free and ready for immediate use in the most sensitive applications. The unique ZymoPURE™ methodology removes the need for slow Part of the ZymoPURE Plasmid Kits collection, the ZymoPURE II Plasmid Maxiprep kit provides the fastest and simplest method available to efficiently isolate up to 3 mg of transfection grade plasmid DNA from E. Utilizing a modified alkaline lysis in conjunction with a novel binding system, the ZymoPURE II Midiprep kit can process up to 50 ml of culture in less than 20 minutes. ZymoPURE 96 Plasmid Miniprep Kit: No: 1 — 5 ml: 1 — 100 µg: 100 — 1,000 ng/µl: ≤ 1 EU/µg: ≤ 200 The ZymoPURE™ Plasmid Miniprep Kit features a spin column-based method for the purification of up to 100 µg of ultra-pure endotoxin-free plasmid DNA in less than 15 minutes. Title: DS1204 Transfection-Grade Plasmid from Miniprep Data Sheet 041423 FINAL. We found that decanting instead of pipetting sometimes even increases yields! 1. The ZymoPURE Plasmid Midiprep kit features a spin column-based method for the purification of up to 400 µg of high-quality plasmid DNA in less than 18 minutes. com. Add ethanol to the Wash 2 concentrate according to bottle 2. ZERO BIAS - scores, article reviews, protocol conditions and more purified plasmid is predominately supercoiled and does not contain RNA or genomic DNA (Fig. ZymoPURE Plasmid Miniprep Kit ; Lab Supplies; DNA Product Brochure; An Exclusive Promo Code; Total Value: $200 - Free for Graduate Students! Choose a Full-Sized Kit: Direct-zol RNA Miniprep (for Tri-zol users) Quick-RNA Miniprep Plus Kit (for non-Tri-zol users) Lab Supplies; RNA Product Brochure The ZymoPURE II™ range from Zymo Research enables the isolation of ultra-pure, vaccine/transfection grade plasmid DNA from E. • Recovery Volume: 10 ul • Plasmid Size: Up to 23 Kb (For plasmids > 15 Kb add elution water or TE and incubate for up to 5 minute before ZymoPURE™ Plasmid Midiprep Kit Catalog Nos. ZymoPURE™ technology uses a modified alkaline lysis method and features ZymoPURE™ Plasmid Miniprep Kit Rapid purification of transfection-grade plasmid DNA from up to 5 ml of overnight E. qxtlcjqpzqdvhdnxmbtwyxoslfqowgrnrgrfuprs